Transgenic Arabidopsis Lines Expressing Gene VI from Cauliflower Mosaic Virus Variants Exhibit a Range of Symptom-Like Phenotypes and Accumulate Inclusion Bodies
| Autor: | C. Geri, Edi Cecchini, Joel J. Milner, Simon N. Covey, Zhen-Hui Gong |
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| Rok vydání: | 1997 |
| Předmět: |
Inclusion Bodies
Genetics biology Physiology Transgene Arabidopsis food and beverages General Medicine Plants Genetically Modified biology.organism_classification Inclusion bodies Virus Microscopy Electron Viral Proteins Phenotype Caulimovirus Gene expression Trans-Activators Arabidopsis thaliana Cauliflower mosaic virus Agronomy and Crop Science Gene |
| Zdroj: | Molecular Plant-Microbe Interactions®. 10:1094-1101 |
| ISSN: | 1943-7706 0894-0282 |
| Popis: | Gene VI of cauliflower mosaic virus (CaMV) is an important determinant of symptom expression during infection. We have constructed a series of transgenic Arabidopsis lines that express gene VI protein (P6) from two CaMV isolates (Bari-1 and Cabb B-JI) that cause mild and severe symptoms, respectively, in Arabidopsis, and from a recombinant virus (Baji-31) with a hybrid gene VI that causes very severe symptoms. From 41 transgenic lines analyzed, 17 showed symptom-like phenotypes that ranged from mild vein chlorosis to severe chlorosis and stunting. P6 levels in trans-genic lines varied from undetectable in the lowest expressors to levels greater than those in CaMV-infected plants. There was a strong correlation between phenotype severity and the level of P6, and with the gene VI origin in the order, Baji-31 > B-JI > Bari-1. This was similar to symptom severity in Arabidopsis infected with the respective CaMV variant. We also found that transgenic P6 accumulated in inclusion bodies that were similar to those found in infected plants but lacking virions. We conclude that expression of P6, in the absence of virus replication, elicits a subset of the host symptom responses normally observed during infection and that the level, sequence, and possibly the form of P6 are important in potentiating the process. |
| Databáze: | OpenAIRE |
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