Matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry as a tool for rapid diagnosis of potentially toxigenic Corynebacterium species in the laboratory management of diphtheria-associated bacteria
| Autor: | Ingrid Huber, Andreas Sing, Stefan Hörmansdorfer, Ulrich Busch, V. Boschert, Sören Schubert, Michael Hogardt, Anja Berger, Regina Konrad |
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| Rok vydání: | 2010 |
| Předmět: |
Epidemiology
Toxigenic Corynebacterium Corynebacterium Mass spectrometry Polymerase Chain Reaction complex mixtures Microbiology Matrix (chemical analysis) Germany Virology Corynebacterium ulcerans medicine Humans Diphtheria Toxin Diphtheria toxin Bacteriological Techniques biology Diphtheria Public Health Environmental and Occupational Health biology.organism_classification MALDI-TOF Mass Spectrometry medicine.disease Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Laboratories Algorithms |
| Zdroj: | Eurosurveillance. 15 |
| ISSN: | 1560-7917 |
| Popis: | The rapid identification of the potentially toxigenic Corynebacterium species, C. diphtheriae, C. ulcerans and C. pseudotuberculosis is essential for diagnosis and treatment of diphtheria and diphtheria-like diseases. We used matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) in comparison with classical microbiological and molecular methods on 116 Corynebacterium strains. All 90 potentially toxigenic Corynebacterium strains collected by the German National Consiliary Laboratory on Diphtheria in a period of more than ten years were correctly identified by MALDI-TOF MS. We propose an algorithm for fast and reliable diagnosis of diphtheria incorporating MALDI-TOF MS, real-time tox PCR and Elek testing. |
| Databáze: | OpenAIRE |
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