Human papillomavirus prevalence and type-distribution in women with cervical lesions: a cross-sectional study in Sri Lanka
| Autor: | Kusuma Gopala, Sanjoy K Datta, Himali Ihalagama, Saman Rohitha, Jing Chen, Anco Molijn, Kanishka Karunaratne, Johannes E. Schmidt, Shailesh Mehta |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Human papillomavirus Cancer Research medicine.medical_specialty Type-distribution Cross-sectional study Uterine Cervical Neoplasms Cervical intraepithelial neoplasia Cohort Studies Carcinoma Adenosquamous Prevalence Genetics medicine Carcinoma Humans Prospective Studies Prospective cohort study Sri Lanka Gynecology Cervical cancer Human papillomavirus 16 Human papillomavirus 18 business.industry Papillomavirus Infections HPV infection virus diseases Cancer Middle Aged medicine.disease female genital diseases and pregnancy complications Cross-Sectional Studies Oncology Carcinoma Squamous Cell Female business Research Article Cohort study |
| Zdroj: | BMC Cancer |
| ISSN: | 1471-2407 |
| DOI: | 10.1186/1471-2407-14-116 |
| Popis: | Background Cervical cancer ranks second among all cancers reported in Sri Lankan women. This study assessed the prevalence and type-distribution of human papillomavirus (HPV) among Sri Lankan women with invasive cervical cancer (ICC) and pre-cancerous lesions. Methods 114 women aged 21 years and above, hospitalized in the National Cancer Institute, Sri Lanka with a diagnosis of ICC or cervical intraepithelial neoplasia (CIN) 2/3 were prospectively enrolled between October 2009 and September 2010 (110430/NCT01221987). The cervical biopsy or excision specimens collected during routine clinical procedures were subjected to histopathological review. DNA was extracted from samples with a confirmed histological diagnosis and was amplified using polymerase chain reaction and HPV DNA was detected using Enzyme Immuno Assay. HPV positive samples were typed using reverse hybridization Line Probe Assay. Results Of the cervical samples collected, 93.0% (106/114) had a histologically confirmed diagnosis of either ICC (98/106) or CIN 2/3 (8/106). Among all ICC cases, squamous cell carcinoma was diagnosed in the majority of women (81.6% [80/98]). HPV prevalence among ICC cases was 84.7% (83/98). The HPV types most commonly detected in ICC cases with single HPV infection (98.8% [82/83]) were HPV-16 (67.3%) and HPV-18 (9.2%). Infection with multiple HPV types was recorded in a single case (co-infection of HPV-16 and HPV-59). Conclusions HPV was prevalent in most women with ICC in Sri Lanka; HPV-16 and HPV-18 were the predominantly detected HPV types. An effective prophylactic vaccine against the most prevalent HPV types may help to reduce the burden of ICC disease. |
| Databáze: | OpenAIRE |
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