SIMKK, a Mitogen-Activated Protein Kinase (MAPK) Kinase, Is a Specific Activator of the Salt Stress–Induced MAPK, SIMK
| Autor: | Aneta Liwosz, Emmanuel Baudouin, László Bögre, Sandra Till, Francesca Cardinale, Christine Siligan, Staffan Eklöf, Irute Meskiene, Andrea Gross, Stefan Kiegerl, Heribert Hirt |
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| Přispěvatelé: | Institute of Microbiology and Genetics, University of Vienna [Vienna] |
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
0106 biological sciences
MAPK/ERK pathway Threonine [SDV]Life Sciences [q-bio] Molecular Sequence Data Plant Science [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology 01 natural sciences Substrate Specificity 03 medical and health sciences Enzyme activator Two-Hybrid System Techniques Amino Acid Sequence Cloning Molecular Phosphorylation Amino Acid Sequence Base Sequence Cloning Protein kinase A Transcription factor ComputingMilieux_MISCELLANEOUS 030304 developmental biology DNA Primers Plant Proteins Mitogen-Activated Protein Kinase Kinases 0303 health sciences Base Sequence Sequence Homology Amino Acid Activator (genetics) Kinase [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Biology Faculty of Science\Biological Science Cell biology [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Enzyme Activation Biochemistry Mitogen-activated protein kinase Molecular DNA Primers Enzyme Activation Medicago sativa/enzymology Mitogen-Activated Protein Kinase Kinases/chemistry/genetics/*metabolism Mitogen-Activated Protein Kinases/chemistry/genetics/*metabolism Molecular Sequence Data Phosphorylation *Plant Proteins Sequence Homology Amino Acid Substrate Specificity Threonine/metabolism Two-Hybrid System Techniques Tyrosine/metabolism biology.protein Tyrosine Mitogen-Activated Protein Kinases 010606 plant biology & botany Medicago sativa Research Article |
| Zdroj: | The Plant cell The Plant cell, American Society of Plant Biologists (ASPB), 2000, 12 (11), pp.2247-2258. ⟨10.1105/tpc.12.11.2247⟩ Plant Cell, 12 (11) |
| ISSN: | 1040-4651 1532-298X |
| DOI: | 10.1105/tpc.12.11.2247⟩ |
| Popis: | In eukaryotes, mitogen-activated protein kinases (MAPKs) play key roles in the transmission of external signals, such as mitogens, hormones, and different stresses. MAPKs are activated by MAPK kinases through phosphorylation of MAPKs at both the threonine and tyrosine residues of the conserved TXY activation motif. In plants, several MAPKs are involved in signaling of hormones, stresses, cell cycle, and developmental cues. Recently, we showed that salt stress-induced MAPK (SIMK) is activated when alfalfa cells are exposed to hyperosmotic conditions. Here, we report the isolation and characterization of the alfalfa MAPK kinase SIMKK (SIMK kinase). SIMKK encodes an active protein kinase that interacts specifically with SIMK, but not with three other MAPKs, in the yeast two-hybrid system. Recombinant SIMKK specifically activates SIMK by phosphorylating both the threonine and tyrosine residues in the activation loop of SIMK. SIMKK contains a putative MAPK docking site at the N terminus that is conserved in mammalian MAPK kinases, transcription factors, and phosphatases. Removal of the MAPK docking site of SIMKK partially compromises but does not completely abolish interaction with SIMK, suggesting that other domains of SIMKK also are involved in MAPK binding. In transient expression assays, SIMKK specifically activates SIMK but not two other MAPKs. Moreover, SIMKK enhances the salt-induced activation of SIMK. These data suggest that the salt-induced activation of SIMK is mediated by the dual-specificity protein kinase SIMKK. |
| Databáze: | OpenAIRE |
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