Overexpression of MicroRNA-216a Suppresses Proliferation, Migration, and Invasion of Glioma Cells by Targeting Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 5
| Autor: | Fei Liang, Yan Zhao, Li Zhang, Junfeng Zhang, Li-li Shi, Hong-Bo Li, Ying-fang Tian, Xu Xi, Hao Xu, Zhao-hua Zhao, Kun Xu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Proliferation Transfection Article Receptors G-Protein-Coupled 03 medical and health sciences 0302 clinical medicine Invasion Cell Movement Cell Line Tumor Glioma microRNA medicine Humans Neoplasm Invasiveness Migration Cell Proliferation Oncogene Brain Neoplasms Cell growth Chemistry Wnt signaling pathway LGR5 General Medicine medicine.disease Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) MicroRNAs 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer research Signal transduction |
| Zdroj: | Oncology Research |
| ISSN: | 0965-0407 |
| DOI: | 10.3727/096504017x14874323871217 |
| Popis: | Increasing studies have suggested that microRNAs (miRNAs) are involved in the development of gliomas. MicroRNA-216a has been reported to be a tumor-associated miRNA in many types of cancer, either as an oncogene or as a tumor suppressor. However, little is known about the function of miR-216a in gliomas. The present study was designed to explore the potential role of miR-216a in gliomas. We found that miR-216a was significantly decreased in glioma tissues and cell lines. Overexpression of miR-216a significantly suppressed the proliferation, migration, and invasion of glioma cells. Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) was identified as a target gene of miR-216a in glioma cells by bioinformatics analysis, dual-luciferase reporter assay, real-time quantitative polymerase chain reaction, and Western blot analysis. Moreover, miR-216a overexpression inhibited the Wnt/β-catenin signaling pathway. The restoration of LGR5 expression markedly reversed the antitumor effect of miR-216a in glioma cells. Taken together, these findings suggest a tumor suppressor role for miR-216a in gliomas, which inhibits glioma cell proliferation, migration, and invasion by targeting LGR5. Our study suggests that miR-216a may serve as a potential therapeutic target for future glioma treatment. |
| Databáze: | OpenAIRE |
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