Chromosomal Aberration Test in Human Lymphocytes

Autor:	G. Obe, Christian Johannes
Rok vydání:	2019
Předmět:	chemistry.chemical_compound Colcemid In vivo Chemistry Cell culture food and beverages Cell cycle Biologie Metaphase In vitro Spindle apparatus Staining Cell biology
Zdroj:	Methods in Molecular Biology ISBN: 9781493996452 Methods in Molecular Biology ISBN: 9781627035286
DOI:	10.1007/978-1-4939-9646-9_6
Popis:	Human peripheral lymphocytes (HPL) are non-cycling primary cells (G0 cells). They are easily collectable by venipuncture. In the presence of suitable culture media and stimulants in vitro HPL enter the cell cycle and divide mitotically. Metaphase-like stages can be arrested using the spindle fiber poison colcemid and prepared on microscopic slides. Following appropriate staining, chromosomal aberrations can be analyzed in the microscope. These aberrations may be induced either in vivo by environmental or occupational influences or in vitro after experimentally controlled manipulations in order to detect or to test the mutagenic potency of various agents.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01ef24e8764e007f769bf24859d709c4 https://doi.org/10.1007/978-1-4939-9646-9_6 Zobrazit plný text záznamu