Characterization, biological evaluation and molecular docking of mulberry fruit pectin
| Autor: | Prashant Singh, Rajesh Kumar, Dinesh Kumar, Devika Srivastava, Vandana Singh, Umesh Kumar, Vijay Kumar Vishvakarma, R. Venkatesh Kumar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030103 biophysics food.ingredient Pectin Science Cell Bacterial growth Microbiology Article 03 medical and health sciences food medicine Humans Food science Cytotoxicity Cancer Multidisciplinary Cytotoxins Chemistry Biological techniques food and beverages Hep G2 Cells Antimicrobial In vitro Computational biology and bioinformatics Anti-Bacterial Agents Molecular Docking Simulation 030104 developmental biology medicine.anatomical_structure Docking (molecular) Cell culture Fruit Pectins Medicine Morus Drug Screening Assays Antitumor HT29 Cells |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-16 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Contemplating the exemplary benefits of pectin on human health, we precisely characterized and evaluated the antibacterial and anticancer activities from purified Mulberry Fruit Pectins (MFP). Here, we tested BR-2 and S-13 varieties of mulberry fruit pectins against six bacterial strains and two human cancer cell lines (HT-29 and Hep G-2), using MIC and an in vitro cell-based assay respectively. The BR-2 mulberry fruit pectin performs superior to S-13 by inhibiting strong bacterial growth (MIC = 500–1000 μg/mL) against tested bacterial strains and cytotoxic activities at the lowest concentration (10 µg/ml) against the Hep G-2 cell line. However, both tested drugs failed to exhibit cytotoxicity on the human colon cancer cell line (HT-29). Based on molecular interaction through docking, pectin binds effectively with the receptors (1e3g, 3t0c, 5czz, 6j7l, 6v40, 5ibs, 5zsy, and 6ggb) and proven to be a promising antimicrobial and anti-cancer agents. The pursuit of unexploited drugs from mulberry fruit pectin will potentially combat against bacterial and cancer diseases. Finally, future perspectives of MFP for the treatment of many chronic diseases will help immensely due to their therapeutic properties. |
| Databáze: | OpenAIRE |
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