Influence of nanoscale-modified apatite-type calcium phosphates on the biofilm formation by pathogenic microorganisms
| Autor: | I. I. Grynyuk, M.S. Slobodyanik, Svitlana Prylutska, Nataliia Strutynska, Vasyliuk Om, Oksana V. Livitska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
staphylococcus aureus
0303 health sciences Microorganism Biofilm chemistry.chemical_element 02 engineering and technology General Chemistry Calcium 021001 nanoscience & nanotechnology Apatite pseudomonas aeruginosa 03 medical and health sciences Chemistry chemistry Chemical engineering visual_art apatite Materials Chemistry visual_art.visual_art_medium biofilm formation nanoparticles 0210 nano-technology Nanoscopic scale QD1-999 030304 developmental biology |
| Zdroj: | Open Chemistry, Vol 19, Iss 1, Pp 39-48 (2021) |
| ISSN: | 2391-5420 |
| Popis: | Nanoparticles (25–50 nm) of chemically modified calcium phosphates Ca10−x−y M ii x Na y (PO4)6−z (CO3) z (OH)2 (M ii – Cu2+, Zn2+) were synthesized via a wet precipitation method at room temperature. The Fourier-transform infrared spectroscopy data confirmed the partial substitution of PO 4 3 − {\text{PO}}_{4}^{3-} → CO 3 2 − {\text{CO}}_{3}^{2-} (B-type) in apatite-type structure. The influence of prepared phosphates on biofilm formation by pathogenic microorganisms was investigated. It was found that the samples Na+, CO 3 2 − {\text{CO}}_{3}^{2-} -hydroxyapatite (HAP) and Na+, Zn2+, CO 3 2 − {\text{CO}}_{3}^{2-} -HAP (5–20 mM) had the highest inhibitory effect on biofilm formation by Staphylococcus aureus strains. The sample Na+, CO 3 2 − {\text{CO}}_{3}^{2-} -HAP had the slight influence on the formation of the biofilm by Pseudomonas aeruginosa, while for the samples Na+, Cu2+, CO 3 2 − {\text{CO}}_{3}^{2-} -HAP and Na+, Zn2+, CO 3 2 − {\text{CO}}_{3}^{2-} -HAP such an effect was not detected. According to transmission electron microscopy data, a correlation between the activity of synthesized apatite-related modified calcium phosphates in the processes of biofilm formation and their ability to adhere to the surface of bacterial cells was established. The prepared samples can be used for the design of effective materials with antibacterial activity for medicine. |
| Databáze: | OpenAIRE |
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