Growth phase-dependent control of transcription start site selection and gene expression by nanoRNAs

Autor:	Josh S. Sharp, Jonathan Livny, Simon L. Dove, Bryce E. Nickels, Seth R. Goldman, Pinal N. Kanabar, Irina O. Vvedenskaya
Rok vydání:	2012
Předmět:	Genetics biology General transcription factor Eukaryotic transcription Response element RNA polymerase II Promoter E-box Ribonucleases Gene Expression Regulation biology.protein RNA Transcription Initiation Site Enhancer Transcription factor II B Research Paper Developmental Biology
Zdroj:	Genes & Development. 26:1498-1507
ISSN:	1549-5477 0890-9369
DOI:	10.1101/gad.192732.112
Popis:	Prokaryotic and eukaryotic RNA polymerases can use 2- to ∼4-nt RNAs, “nanoRNAs,” to prime transcription initiation in vitro. It has been proposed that nanoRNA-mediated priming of transcription can likewise occur under physiological conditions in vivo and influence transcription start site selection and gene expression. However, no direct evidence of such regulation has been presented. Here we demonstrate in Escherichia coli that nanoRNAs prime transcription in a growth phase-dependent manner, resulting in alterations in transcription start site selection and changes in gene expression. We further define a sequence element that determines, in part, whether a promoter will be targeted by nanoRNA-mediated priming. By establishing that a significant fraction of transcription initiation is primed in living cells, our findings contradict the conventional model that all cellular transcription is initiated using nucleoside triphosphates (NTPs) only. In addition, our findings identify nanoRNAs as a previously undocumented class of regulatory small RNAs that function by being directly incorporated into a target transcript.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01cfe56cf6cd2eef7d6a9e013fa63321 https://doi.org/10.1101/gad.192732.112 Zobrazit plný text záznamu