Sex hormones modulate salbutamol-elicited long-term relaxation in isolated bovine tracheal strips
| Autor: | Manuel Sánchez, María Pipa, Javier Granda, María José García de Boto, Begoña Cantabrana, Ana Coto, Javier Bordallo, Lorena Suárez |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Muscle Relaxation Adrenergic beta-Antagonists Adrenergic Bronchi In Vitro Techniques Epithelium Sex hormone-binding globulin Internal medicine medicine Cyclic AMP Animals Albuterol Testosterone Receptor Pharmacology biology Relaxation (psychology) Muscle Smooth General Medicine Androgen Propranolol Flutamide Trachea Endocrinology 3' 5'-Cyclic-AMP Phosphodiesterases Muscle Tonus biology.protein Salbutamol Carbachol Cattle Receptors Adrenergic beta-2 Airway medicine.drug Hormone |
| Zdroj: | Pharmacology. 87(5-6) |
| ISSN: | 1423-0313 |
| Popis: | Sex hormones are of interest regarding gender differences in the clinical manifestations of airway diseases. No conclusive data are available on the sex hormone modulation of β-adrenoceptor-mediated responses on airways. To this aim, isolated preparations of bovine trachea were used to establish the sex hormone influence on salbutamol-elicited relaxation. This had 2 components, a full acute relaxation followed by a loss of efficacy, close to half of the effect. The remaining half was reverted by the β-blocker, propranolol. The loss of salbutamol-elicited relaxation might reflect the receptor desensitization, as shown by the lack of effect by subsequent administration of salbutamol, and the decrease in the immunostaining of β2-adrenoceptors. Sex hormones differently modified the salbutamol-elicited response. Testosterone, but not other androgens or estradiol, had a synergic effect, facilitating the acute relaxation and decreasing the loss of spasmolytic effect, associated with an increase in the latency of desensitization and a decrease in the time taken to reach long-term steady-state tone. These effects, not modified by the antiandrogen flutamide or epithelium removal, seem to be independent of a modulation of β2-adrenoceptor desensitization. Testosterone also relaxed preparations with desensitized β-adrenoceptor. Therefore, testosterone modulates tracheal smooth muscle tone, facilitating bronchodilation caused by β2-adrenoceptor agonists which might be of pharmacological interest. |
| Databáze: | OpenAIRE |
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