Lipocalin-2-mediated upregulation of various antioxidants and growth factors protects bone marrow-derived mesenchymal stem cells against unfavorable microenvironments
| Autor: | Raheleh Halabian, Ali Jahanian-Najafabadi, Hossein Abdul Tehrani, Mehryar Habibi Roudkenar |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Programmed cell death
Cellular differentiation Bone Marrow Cells Biology Biochemistry Antioxidants Rats Sprague-Dawley Cell therapy Lipocalin-2 Proto-Oncogene Proteins Cell Adhesion medicine Animals Humans Cell adhesion Cell Proliferation Original Paper Superoxide Dismutase Cell growth Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Hep G2 Cells Cell Biology Lipocalins Rats Up-Regulation Cell biology Transplantation medicine.anatomical_structure Cellular Microenvironment Intercellular Signaling Peptides and Proteins Bone marrow Heme Oxygenase-1 Acute-Phase Proteins |
| Zdroj: | Cell Stress and Chaperones. 18:785-800 |
| ISSN: | 1466-1268 1355-8145 |
| Popis: | Despite many advantages of mesenchymal stem cells (MSCs) that make them suitable for cell therapy purposes, their therapeutic application has been limited due to their susceptibility to several stresses (e.g., nutrient-poor environment, oxidative stress, and hypoxic and masses of cytotoxic factors) to which they are exposed during their preparation and following transplantation. Hence, reinforcing MSCs against these stresses is a challenge for both basic and clinician scientists. Recently, much attention has been directed toward equipping MSCs with cytoprotective factors to strengthen them against unfavorable microenvironments. Here, we engineered MSCs with lipocalin 2 (Lcn2), a cytoprotective factor that is naturally induced following exposure of cells to stresses imposed by the microenvironment. Lcn2 overexpression not only did not interfere with the multidifferentiation capacity of the MSCs but also granted many protective properties to them. Lcn2 potentiated MSCs to withstand oxidative, hypoxia, and serum deprivation (SD) conditions via antagonizing their induced cytotoxicity and apoptosis. Adhesion rate of MSCs to coated culture plates was also enhanced by Lcn2 overexpression. In addition, Lcn2 induced antioxidants and upregulated some growth factors in MSCs. Our findings suggested a new strategy for prevention of graft cell death in MSC-based cell therapy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink