Epigenetic control of gonadal aromatase (cyp19a1) in temperature-dependent sex determination of red-eared slider turtles
| Autor: | David Crews, Alvin Buemio, Randy A. Chu, Mozhgon Vafaee, Yuiko Matsumoto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
5' Flanking Region Codon Initiator lcsh:Medicine Biochemistry Epigenesis Genetic 0302 clinical medicine Molecular cell biology Morphogenesis Aromatase Promoter Regions Genetic lcsh:Science Base Pairing Conserved Sequence 0303 health sciences Multidisciplinary Sexual Differentiation biology Temperature-dependent sex determination Temperature Turtles Nucleic acids CpG site Organ Specificity DNA methylation Epigenetics Female Transcription Initiation Site DNA modification Research Article endocrine system TATA box 5' flanking region Molecular Sequence Data 03 medical and health sciences Sequence Homology Nucleic Acid Genetics Animals Gonads Zebra finch Biology 030304 developmental biology Binding Sites Base Sequence lcsh:R Promoter DNA Sex Determination DNA Methylation Sex Determination Processes Molecular biology Hormones Protein Biosynthesis biology.protein CpG Islands lcsh:Q Gene expression 030217 neurology & neurosurgery Developmental Biology Transcription Factors |
| Zdroj: | PLoS ONE, Vol 8, Iss 6, p e63599 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | In the red-eared slider turtle (Trachemys scripta), a species with temperature-dependent sex determination (TSD), the expression of the aromatase gene during gonad development is strictly limited to the female-producing temperature. The underlying mechanism remains unknown. In this study, we identified the upstream 5′-flanking region of the aromatase gene, gonad-specific promoter, and the temperature-dependent DNA methylation signatures during gonad development in the red-eared slider turtle. The 5′-flanking region of the slider aromatase exhibited sequence similarities to the aromatase genes of the American alligator, chicken, quail, and zebra finch. A putative TATA box was located 31 bp upstream of the gonad-specific transcription start site. DNA methylation at the CpG sites between the putative binding sites of the fork head domain factor (FOX) and vertebrate steroidogenic factor 1 (SF1) and adjacent TATA box in the promoter region were significantly lower in embryonic gonads at the female-producing temperature compared the male-producing temperature. A shift from male- to female-, but not from female- to male-, producing temperature changed the level of DNA methylation in gonads. Taken together these results indicate that the temperature, particularly female-producing temperature, allows demethylation at the specific CpG sites of the promoter region which leads the temperature-specific expression of aromatase during gonad development. |
| Databáze: | OpenAIRE |
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