Implication of cell culture methods and biases on UV inactivation of viruses
| Autor: | Absar, Alum, Zhe, Zhao, Mahmut S, Ersan, Thorsten, Mewes, Michael, Barnes, Paul, Westerhoff, Morteza, Abbaszadegan |
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| Rok vydání: | 2022 |
| Předmět: |
History
Polymers and Plastics Nitrogen Ultraviolet Rays Cell Culture Techniques Vitamins Industrial and Manufacturing Engineering Culture Media Disinfection Oxygen Bias Nucleic Acids Virology Viruses Humans Virus Inactivation Business and International Management Coloring Agents Reactive Oxygen Species |
| Zdroj: | Journal of Virological Methods. 309:114610 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2022.114610 |
| Popis: | Inactivation of human respiratory viruses in air and on surfaces is important to control their spread. Exposure to germicidal ultraviolet (UV-C) light damages viral nucleic acid rendering them non-infectious. Most of the recent viral inactivation studies have not considered potential artifacts caused by interactions between UV-C light and culture media used to suspend and deposit virus on surfaces. We show that the reactive oxygen and nitrogen species (ROS and RNS) form when commonly used virus culture media is exposed to 265 nm irradiation from light emitting diodes (LEDs) at UV-C doses (4 or 40 mJ/cm |
| Databáze: | OpenAIRE |
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