Enteral nutrition ameliorates the symptoms of Crohn's disease in mice via activating special pro-resolving mediators through innate lymphoid cells
| Autor: | Chen Ye, Di Zhao, Qiyi Chen, Shaoyi Zhang, Bo Yang, Xiaoqiong Lv |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Immunology
Perforation (oil well) innate lymphoid cells Microbiology Flow cytometry chemistry.chemical_compound Mice Enteral Nutrition Western blot Crohn Disease medicine Animals Lymphocytes Molecular Biology Survival rate Crohn's disease medicine.diagnostic_test business.industry Tumor Necrosis Factor-alpha Innate lymphoid cell Cell Biology Original Articles RC581-607 medicine.disease lipoxygenase Immunity Innate Baicalein special pro-resolving mediators Infectious Diseases Parenteral nutrition chemistry Immunologic diseases. Allergy business |
| Zdroj: | Innate Immunity, Vol 27 (2021) Innate Immunity |
| ISSN: | 1753-4267 1753-4259 |
| Popis: | Crohn's disease activates the inflammatory reactions to induce intestinal disorders. Enteral nutrition (EN) could exert general immunomodulatory effects. Cecal ligation and perforation (CLP) surgery was utilized to establish Crohn's disease mice models. Survival analysis, hematoxylin-eosin staining, flow cytometry, ELISA, Western blot and liquid chromatography-tandem MS were applied. Baicalein was added to inhibit lipoxygenases. The survival rate was restored and inflammatory injury, exudate neutrophils in peritoneal lavage and serum levels of IL-6 and TNF-α were ameliorated by EN treatment as compared with CLP treatment. EN also increased ILC-3 content, 5/15-LOX level and RvD1-RvD5 in peritoneal lavage. Baicalein reversed all the detected effects of EN except ILC-3 content. EN could activate special pro-resolving mediators (SPMs) through ILCs to mitigate injuries of Crohn's disease. |
| Databáze: | OpenAIRE |
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