A label-free biosensor for selective detection of Gram-negative bacteria based on the oxidase-like activity of cupric oxide nanoparticles

Autor:	Suqi Deng, Yiwen Tu, Li Fu, Jia Liu, Li Jia
Rok vydání:	2022
Předmět:	Oxygen Bacteria Gram-Negative Bacteria Nanoparticles Biosensing Techniques Oxidoreductases Copper Analytical Chemistry
Zdroj:	Mikrochimica acta. 189(12)
ISSN:	1436-5073
Popis:	A label-free biosensor based on cupric oxide (CuO) nanoparticles was constructed for the selective detection of Gram-negative bacteria. CuO possesses oxidase-like activity and can catalyze the oxidation of o-phenylenediamine (OPD) to produce oxidized OPD, which has a fluorescence emission at 573 nm under excitation at 423 nm. The mechanism study suggests that the oxygen vacancies of CuO can activate the dissolved oxygen to form superoxide anions, which in turn oxidize OPD. Gram-negative bacteria can reduce part of Cu(II) in CuO to Cu(I) based on their copper homeostasis system, thus inhibiting the oxidation of OPD and decreasing the fluorescence intensity of the catalytic system. This principle was utilized to construct a biosensor to realize the selective detection of Gram-negative bacteria successfully. The biosensor exhibited a good linear correlation toward the logarithm concentration of three Gram-negative bacteria with R
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01727967cae9fe1e09f6da892aa41c34 https://pubmed.ncbi.nlm.nih.gov/36434468 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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