Essential genome of Pseudomonas aeruginosa in cystic fibrosis sputum
Autor: | Gregory C. Palmer, Aimee K. Wessel, Keith H. Turner, Marvin Whiteley, Justine L. Murray |
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Rok vydání: | 2015 |
Předmět: |
Cystic Fibrosis
Riboflavin Biotin Biology medicine.disease_cause Genome Cystic fibrosis Polymerase Chain Reaction Pantothenic Acid Microbiology law.invention chemistry.chemical_compound Species Specificity law medicine Humans Computer Simulation Pseudomonas Infections Gene Lung Polymerase chain reaction Growth medium Multidisciplinary Pseudomonas aeruginosa Stem Cells Sputum Transposon Sequencing Biological Sciences medicine.disease chemistry Wounds and Injuries medicine.symptom Monte Carlo Method Genome Bacterial |
Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 112(13) |
ISSN: | 1091-6490 |
Popis: | Defining the essential genome of bacterial pathogens is central to developing an understanding of the biological processes controlling disease. This has proven elusive for Pseudomonas aeruginosa during chronic infection of the cystic fibrosis (CF) lung. In this paper, using a Monte Carlo simulation-based method to analyze high-throughput transposon sequencing data, we establish the P. aeruginosa essential genome with statistical precision in laboratory media and CF sputum. Reconstruction of the global requirements for growth in CF sputum compared with defined growth conditions shows that the latter requires several cofactors including biotin, riboflavin, and pantothenate. Comparison of P. aeruginosa strains PAO1 and PA14 demonstrates that essential genes are primarily restricted to the core genome; however, some orthologous genes in these strains exhibit differential essentiality. These results indicate that genes with similar molecular functions may have distinct genetic roles in different P. aeruginosa strains during growth in CF sputum. We also show that growth in a defined growth medium developed to mimic CF sputum yielded virtually identical fitness requirements to CF sputum, providing support for this medium as a relevant in vitro model for CF microbiology studies. |
Databáze: | OpenAIRE |
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