Synergistic activity of chlorhexidine and synoeca‐MP peptide against Pseudomonas aeruginosa
Autor: | Ingrid Aquino Amorim, Jeeser Alves de Almeida, Stella Maris de Freitas Lima, Octavio L. Franco, Tássio F. Cunha, Ana Paula C. Cantuária, Elaine M. G. L. Dantas, Taia Maria Berto Rezende |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Minimum bactericidal concentration Physiology Pseudomonas aeruginosa Clinical Biochemistry Biofilm Context (language use) Cell Biology Antimicrobial medicine.disease_cause Nitric oxide Microbiology 03 medical and health sciences chemistry.chemical_compound Minimum inhibitory concentration 030104 developmental biology 0302 clinical medicine chemistry 030220 oncology & carcinogenesis medicine Cytotoxicity |
Zdroj: | Journal of Cellular Physiology. 234:16068-16079 |
ISSN: | 1097-4652 0021-9541 |
Popis: | This study aims to evaluate the in vitro antimicrobial and immunomodulatory activities and cytotoxicity of chlorhexidine (CHX) and synoeca-MP peptide alone or in combination against Pseudomonas aeruginosa. The antimicrobial property was evaluated by the determination of minimal inhibitory concentration, minimum bactericidal concentration, and planktonic bacteria and biofilm inhibition. Immunomodulatory activity was determined by enzyme-linked immunosorbent assay and nitric oxide production by the Griess reaction method. According to the results, synoeca-MP combined with CHX demonstrated antimicrobial effectiveness compared with its isolated use, in addition to immunomodulatory activity (upregulating MPC-1 and tumor necrosis factor-α and downregulating nitric oxide and interleukin-10). In this context, it is expected that the substances, together, could be capable of controlling bacterial infection and dissemination, besides potentiating macrophages' immune response against the studied microorganism. Moreover, reducing the CHX concentration by the addition of synoeca-MP peptide may, in a beneficial way, minimize the undesirable effects of both, CHX and synoeca-MP in a clinical setting. |
Databáze: | OpenAIRE |
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