Oligopotent Mesenchymal Stem Cell-Like Clone Becomes Multinucleated Following Phorbol Ester, TPA Stimulation
| Autor: | Hajime Sawada, Michiyo Esaki, Tatsuo Maejima, Keiichiro Yoshida, Michio Ono |
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| Rok vydání: | 2007 |
| Předmět: |
Cell Nucleus
Osteoblasts Histology Tight junction Cellular differentiation Mesenchymal stem cell Clone (cell biology) Cell Differentiation Mesenchymal Stem Cells Biology Cell junction In vitro Cell Line Rats Cell biology Intercellular Junctions Phenotype Multinucleate Cell culture Phorbol Esters Adipocytes Animals Anatomy Ecology Evolution Behavior and Systematics Biotechnology |
| Zdroj: | The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology. 290:1256-1267 |
| ISSN: | 1932-8494 1932-8486 |
| DOI: | 10.1002/ar.20590 |
| Popis: | We established a mesenchymal stem cell clone, 5F9A, from rat bone marrow substrate adherent cells by repeated limiting dilutions. The cells have a fibroblastic shape and form intimate contacts with adjacent cells with interdigitations and junctions similar to adherence and tight junctions in a semi-confluent culture. Analysis of the phenotypes of these cells by RT-PCR and FACS demonstrated that they resembled mesenchymal stem cells, and the cells could differentiate into adiopocytes and osteoblasts under appropriate conditions in vitro showing their oligopotency. Furthermore, the cells were induced to become multinuclear cells by TPA (12-o-tetradecanoylphorbol 13-acetate) stimulation. |
| Databáze: | OpenAIRE |
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