Multifocus microscopy with precise color multi-phase diffractive optics applied in functional neuronal imaging
| Autor: | Joan Pulupa, Lei Chen, Sara Abrahamsson, Bassam Hajj, Liya Yu, Mohamed El Beheiry, Xavier Darzacq, Marcelo Davanco, Mustafa Mir, Laura Oudjedi, Jan Wisniewski, Jean-Bernard Fiche, Marcelo Nollmann, Cornelia I. Bargmann, J. Alexander Liddle, Carl Wu, Rob Ilic, Xin Jin, Christine E. Cho, Maxime Dahan, Timothée Lionnet, Brian Mehl |
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| Přispěvatelé: | Rockefeller University [New York], National Institute of Standards and Technology [Gaithersburg] (NIST), Janelia Farm Research Campus, Howard Hughes Medical Institute (HHMI), Centre de Biochimie Structurale [Montpellier] (CBS), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physico-Chimie-Curie (PCC), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC), Lawrence Berkeley National Laboratory [Berkeley] (LBNL), HAL UPMC, Gestionnaire, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Photon Materials science Image quality [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging (110.4190) Multiple imaging (180.2520) Fluorescence microscopy (260.5430) Polarization Bioengineering Optical Physics Grating 01 natural sciences Three-dimensional microscopy Article 010309 optics 03 medical and health sciences Biological specimen Optics Affordable and Clean Energy Polarization 0103 physical sciences Microscopy Diffractive optics Multiple imaging Fluorescence microscopy business.industry Ranging Materials Engineering (050.1970) Diffractive optics Frame rate Atomic and Molecular Physics and Optics 030104 developmental biology Nanolithography [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging (180.6900) Three-dimensional microscopy Generic health relevance business Biotechnology |
| Zdroj: | Biomedical optics express Biomedical optics express, Optical Society of America-OSA Publishing, 2016, 7 (3), pp.855-869. ⟨10.1364/BOE.7.000855⟩ Biomedical optics express, 2016, 7 (3), pp.855-869. ⟨10.1364/BOE.7.000855⟩ Biomedical optics express, vol 7, iss 3 |
| ISSN: | 2156-7085 |
| DOI: | 10.1364/BOE.7.000855⟩ |
| Popis: | International audience; Multifocus microscopy (MFM) allows high-resolution instantaneous three-dimensional (3D) imaging and has been applied to study biological specimens ranging from single molecules inside cells nuclei to entire embryos. We here describe pattern designs and nanofabrication methods for diffractive optics that optimize the light-efficiency of the central optical component of MFM: the diffractive multifocus grating (MFG). We also implement a " precise color " MFM layout with MFGs tailored to individual fluorophores in separate optical arms. The reported advancements enable faster and brighter volumetric time-lapse imaging of biological samples. In live microscopy applications, photon budget is a critical parameter and light-efficiency must be optimized to obtain the fastest possible frame rate while minimizing photodamage. We provide comprehensive descriptions and code for designing diffractive optical devices, and a detailed methods description for nanofabrication of devices. Theoretical efficiencies of reported designs is ≈90% and we have obtained efficiencies of > 80% in MFGs of our own manufacture. We demonstrate the performance of a multi-phase MFG in 3D functional neuronal imaging in living C. elegans. |
| Databáze: | OpenAIRE |
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