Heterogeneity of the gut microbiome in mice: guidelines for optimizing experimental design
| Autor: | Peter Vandenabeele, Martine De Vos, Debby Laukens, Brigitta M. Brinkman, Jeroen Raes |
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| Přispěvatelé: | Department of Bio-engineering Sciences, Microbiology, Normark, Birgitta Henriques |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
DIET-INDUCED OBESITY INNATE LYMPHOID-CELLS microbiome Computational biology Review Article Biology Gut flora ENVIRONMENTAL-FACTORS Microbiology animal facility GEL-ELECTROPHORESIS DGGE 03 medical and health sciences Human health INTESTINAL MICROBIOTA microbiota Animals Humans Microbiome confounding factors BALB/C MICE Gastrointestinal Microbiome Biology and Life Sciences biology.organism_classification Intervention studies Gut microbiome animal models 3. Good health 030104 developmental biology Infectious Diseases ULCERATIVE-COLITIS Research Design Immunology Models Animal INFLAMMASOME ACTIVATION IMMUNE-SYSTEM LABORATORY MICE Intestinal bacteria Microbiota composition |
| Zdroj: | FEMS Microbiology Reviews FEMS MICROBIOLOGY REVIEWS |
| ISSN: | 0168-6445 |
| Popis: | Targeted manipulation of the gut flora is increasingly being recognized as a means to improve human health. Yet, the temporal dynamics and intra- and interindividual heterogeneity of the microbiome represent experimental limitations, especially in human cross-sectional studies. Therefore, rodent models represent an invaluable tool to study the host–microbiota interface. Progress in technical and computational tools to investigate the composition and function of the microbiome has opened a new era of research and we gradually begin to understand the parameters that influence variation of host-associated microbial communities. To isolate true effects from confounding factors, it is essential to include such parameters in model intervention studies. Also, explicit journal instructions to include essential information on animal experiments are mandatory. The purpose of this review is to summarize the factors that influence microbiota composition in mice and to provide guidelines to improve the reproducibility of animal experiments. Given the unmet need for standardizing the experimental work flow related to gut microbial research in animals, guidelines are required to isolate true effects from confounding factors. |
| Databáze: | OpenAIRE |
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