Synthesis of Bacterial Flagella: Chromosomal Synchrony and Flagella Synthesis
| Autor: | Melvin I. Simon, Geoffrey F. Grant, D. Van Alstyne |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Spores
biology Cell division Structural gene Chromosome Mapping Chromosome Genetics and Molecular Biology Locus (genetics) Bacillus subtilis Chromosomes Bacterial Cell cycle Flagellum biology.organism_classification Microbiology Molecular biology Bacterial Proteins Flagella Molecular Biology Gene Cell Division Caltech Library Services |
| Zdroj: | Journal of Bacteriology. 100:283-287 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.100.1.283-287.1969 |
| Popis: | Synchronous cultures of Bacillus subtilis 168 M were obtained from light-density spores germinated at 46 C and grown at 37 C. This procedure synchronizes both cell division and chromosome replication. The chromosome synchrony was demonstrated by using transformation to measure changes in marker frequency during the cell cycle. The synthesis of two enzymes and of bacterial flagellar protein was also followed. All of the proteins were found to be synthesized continuously with an abrupt doubling in the rate of synthesis at a specific time in the cell cycle. The time at which the doubling occurred for each enzyme corresponded to the time at which the structural gene for the enzyme was replicated. The doubling of the rate of flagella synthesis corresponded to the time of replication of the hisA1 gene. We conclude that the genetic locus for the factors involved in the rate-limiting steps in flagella synthesis are located on the genetic map near the hisA1 locus. |
| Databáze: | OpenAIRE |
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