Amycolatopsis umgeniensis sp. nov., isolated from soil from the banks of the Umgeni River in South Africa
| Autor: | Clifford Omorogie, Shuk-Kwan Cheung, Gareth J. Everest, Candice M. Goodwin, Paul R. Meyers, Andrew E. Cook, Marilize Le Roes-Hill |
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| Rok vydání: | 2012 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Amycolatopsis DNA Ribosomal Microbiology South Africa Bacterial Proteins Phylogenetics RNA Ribosomal 16S Actinomycetales Botany Cluster Analysis Molecular Biology Phylogeny Soil Microbiology Phylogenetic tree biology Strain (biology) Nucleic Acid Hybridization DNA Restriction Enzymes Sequence Analysis DNA General Medicine biology.organism_classification 16S ribosomal RNA Amycolatopsis umgeniensis Bacterial Typing Techniques Genetic distance DNA Gyrase Amycolatopsis alba |
| Zdroj: | Antonie van Leeuwenhoek. 103:673-681 |
| ISSN: | 1572-9699 0003-6072 |
| DOI: | 10.1007/s10482-012-9851-7 |
| Popis: | A novel member of the genus Amycolatopsis was isolated from soil collected from the banks of the Umgeni River, KwaZulu Natal province, South Africa. The strain, designated UM16(T), grouped with the type strains of Amycolatopsis alba, Amycolatopsis coloradensis and Amycolatopsis thailandensis by 16S rRNA gene based phylogeny. Genetic distance values, based on the gyrB and recN genes, between strain UM16(T) and its closest relatives were all above the threshold values of 0.02 and 0.04, respectively, that have been proposed to distinguish Amycolatopsis type strains. DNA-DNA hybridisation experiments confirmed that strain UM16(T) represents a unique genomic species, sharing 18.4 ± 5.1, 16.2 ± 1.8 and 45.8 ± 8.9 % DNA relatedness to the type strains of A. alba, A. coloradensis and A. thailandensis, respectively. The physiological, phylogenetic and DNA-relatedness data support the description of strain UM16(T) as the type strain of a novel species, for which the name Amycolatopsis umgeniensis sp. nov. is proposed (= DSM 45272(T) = NRRL B-24724(T)). |
| Databáze: | OpenAIRE |
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