Palmitoyl ascorbic acid 2-glucoside has the potential to protect mammalian cells from high-LET carbon-ion radiation
| Autor: | Alexis H. Haskins, Dylan J. Buglewicz, Akira Fujimori, Takamitsu A. Kato, Hirokazu Hirakawa, Yasushi Aizawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
DNA Repair DNA damage Cell Survival Lipoylation lcsh:Medicine Heavy Ion Radiotherapy Radiation-Protective Agents Ascorbic Acid CHO Cells Protective Agents Article Glycerides 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cricetulus Glucoside Glucosides Glycerol Animals Linear Energy Transfer lcsh:Science Ions Multidisciplinary biology Chinese hamster ovary cell lcsh:R DNA Ascorbic acid biology.organism_classification 030104 developmental biology chemistry Biochemistry Gamma Rays 030220 oncology & carcinogenesis Micronucleus test lcsh:Q DNA Damage |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-7 (2018) |
| ISSN: | 2045-2322 |
| Popis: | DMSO, glycerol, and ascorbic acid (AA) are used in pharmaceuticals and known to display radioprotective effects. The present study investigates radioprotective properties of novel glyceryl glucoside, ascorbic acid 2-glucoside, glyceryl ascorbate, and palmitoyl ascorbic acid 2-glucoside (PA). Gamma-rays or high-LET carbon-ions were irradiated in the presence of tested chemicals. Lambda DNA damage, cell survival, and micronuclei formation of CHO cells were analyzed to evaluate radioprotective properties. Radiation-induced Lambda DNA damage was reduced with chemical pre-treatment in a concentration-dependent manner. This confirmed tested chemicals were radical scavengers. For gamma-irradiation, enhanced cell survival and reduction of micronuclei formation were observed for all chemicals. For carbon-ion irradiation, DMSO, glycerol, and PA displayed radioprotection for cell survival. Based on cell survival curves, protection levels by PA were confirmed and comparable between gamma-rays and high-LET carbon-ions. Micronuclei formation was only decreased with AA and a high concentration of glycerol treatment, and not decreased with PA treatment. This suggests that mechanisms of protection against high-LET carbon-ions by PA can differ from normal radical scavenging effects that protect DNA from damage. |
| Databáze: | OpenAIRE |
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