Expression analysis of defense-related genes in cucumber (Cucumis sativus L.) against Phytophthora melonis
| Autor: | Maryam Golabadi, Ahmad Reza Golparvar, Lida Hashemi, Mehdi Nasr-Esfahani |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Phytophthora Damping off Lipoxygenase Biology Plant disease resistance Genes Plant Microbiology 03 medical and health sciences 0302 clinical medicine Genotype Gene expression Genetics Molecular Biology Gene Pathogen Disease Resistance Plant Proteins Inoculation Chitinases General Medicine biology.organism_classification 030104 developmental biology 030220 oncology & carcinogenesis Cucumis sativus Cucumis Functional genomics Transcription Factors |
| Zdroj: | Molecular biology reports. 47(7) |
| ISSN: | 1573-4978 |
| Popis: | Phytophthora melonis is the causal agent of damping-off or crown rot, one of the most destructive cucumber diseases that causes severe economic losses in Iran and some other parts of the world. Despite intense research efforts made in the past years, no permanent cure currently exists for this disease. With the aim to understand the molecular mechanisms of defense against P. melonis, root collars and leaves of four cucumber genotypes consisting of resistant Ramezz; moderately resistant Baby and very susceptible Mini 6-23 and Extrem, were monitored for quantitative gene expression analysis of five antifungal and/or anti-oomycete genes (CsWRKY20, CsLecRK6.1, PR3, PR1-1a and LOX1) at three points after inoculation with P. melonis. The gene expression analysis indicated that P. melonis strongly enhanced the expression of these genes after inoculation in both leaves and root collars. Further, not only the transcript levels of these genes were significantly higher in the resistant and moderately resistance genotypes, but also the time point of the highest relative expression ratio for the five genes was different in the four cucumber genotypes. CsWRKY20 and PR3 showed the maximum expression in Ramezz at 48 hours post inoculation (hpi) while CsLecRK6.1, and LOX1 showed the highest expression at 72 hpi. In addition, PR1-1a showed the maximum expression in the Baby at 72 hpi. Root collars responded faster than leaves and some responses were more strongly up-regulated in root collars than in leaves. The genes found to be involved in disease resistance in two different organs of cucumber after pathogen infection. The results suggest that increased expression of these genes led to activation of defense pathways and could be responsible for a reduced P. melonis colonization capacity in Ramezz and Baby. Overall, this work represents a valuable resource for future functional genomics studies to unravel the molecular mechanisms of C. sativus- P. melonis interaction. |
| Databáze: | OpenAIRE |
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