Effectiveness of mesenchymal stems cells cultured by hanging drop vs. conventional culturing on the repair of hypoxic-ischemic-damaged mouse brains, measured by stemness gene expression
| Autor: | Hui Zhang, Yongli Lou, Laijun Song, Dewei Guo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Hypoxic ischemic General Immunology and Microbiology QH301-705.5 General Neuroscience Drop (liquid) Mesenchymal stem cell Biology brain injury Molecular biology General Biochemistry Genetics and Molecular Biology Cell biology 03 medical and health sciences msc stemness 030104 developmental biology Gene expression Biology (General) General Agricultural and Biological Sciences |
| Zdroj: | Open Life Sciences, Vol 11, Iss 1, Pp 519-523 (2016) |
| ISSN: | 2391-5412 |
| Popis: | In this study, we investigated the therapeutic effects of Human Mesenchymal Stem Cells (hMSCs) cultured by hanging drop and conventional culturing methods on cerebellar repair in hypoxic-ischemic (HI) brain injured mice. Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to analyze the expression levels of three stemness genes, Oct4, Sox2 and Nanog, and the migration related gene CXCR4. MSC prepared by hanging drop or conventional techniques were administered intranasally to nine day old mice, and analyzed by MRI at day 28. Results indicate that the MSCs, especially the hanging drop cultured MSCs, significantly improved the mice’s cerebellar damage repair. MSCs derived from the hanging drop culture were smaller than those from the conventional culture. The gene expression levels were significantly increased for the MSCs derived from the hanging drop culture. The mechanism might relate to the fact that the hanging drop cultured MSCs can be kept in an undifferentiated state, resulting in its higher expression level of migration receptor of CXCR4. |
| Databáze: | OpenAIRE |
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