Purification, characterization and comparison with mammalian SCP2 of a chicken SCP2-like protein
| Autor: | M.P. Reinhart, T. Foglia, S.J. Avart |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
animal structures
Physiology Blotting Western Molecular Sequence Data Biology Biochemistry Chromatography Affinity HSPA2 Animals Bile Amino Acid Sequence Molecular Biology Peptide sequence Plant Proteins SCP2 Molecular mass General Medicine Sterol Rats Sterols Sterol carrier protein Liver Homogeneous biology.protein Cattle Electrophoresis Polyacrylamide Gel Antibody Carrier Proteins Chickens |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 100:243-248 |
| ISSN: | 0305-0491 |
| DOI: | 10.1016/0305-0491(91)90368-n |
| Popis: | 1. 1. Three proteins have been isolated from chicken ( Gallus domesticus ) liver that bind antibodies directed against authentic rat sterol carrier protein 2 (SCP 2 ) and have similar molecular mass to the three major immunoreactive rat liver protein (12 kDa, 30–36 kDa, 55–60 kDa). 2. 2. Bile from both chicken and rat contains the high molecular mass immunoreactive species. 3. 3. The chicken 12 kDa SCP 2 -like protein purifies similarly to rat SCP 2 but the homogeneous chicken SCP 2 -like protein is dissimilar in amino acid composition and N- terminal amino acid sequence. 4. 4. The activity of chicken SCP 2 -like protein differs from rat SCP 2 in that it was consistent with fusion (transfer of both polar surface and non-polar core lipids) rather than transfer of polar lipids only. |
| Databáze: | OpenAIRE |
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