Regulation of juvenile hormone biosynthesis in Heliothis virescens by Manduca sexta allatotropin
| Autor: | Asoka Srinivasan, Sonny B. Ramaswamy, Anna Rachinsky |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Physiology Molecular Sequence Data Neuropeptide Biology Biochemistry chemistry.chemical_compound Vitellogenin BAPTA Internal medicine Manduca medicine Animals Amino Acid Sequence Heliothis virescens Sequence Homology Amino Acid Neuropeptides General Medicine biology.organism_classification Juvenile Hormones Endocrinology chemistry Manduca sexta Insect Science Insect Hormones Juvenile hormone biology.protein Calcium Female Corpus allatum Intracellular |
| Zdroj: | Archives of insect biochemistry and physiology. 54(3) |
| ISSN: | 0739-4462 |
| Popis: | In Heliothis virescens, reproduction is strictly dependent on juvenile hormone (JH). In females, mating induces a sharp increase in JH titers, which stimulates increased vitellogenin biosynthesis and higher rates of egg production. JH biosynthesis is presumably stimulated by production and/or release of stimulatory neuropeptides such as allatotropins. There is evidence that allatotropin of H. virescens may be structurally related to Manduca sexta allatotropin (Manse-AT). In a radiochemical in vitro assay, synthetic Manse-AT stimulated JH biosynthesis by corpora allata (CA) of virgin H. virescens females in a dose-dependent manner, but had no effect on CA activity in H. virescens males. In females, the CA showed a transient increase in sensitivity to Manse-AT shortly after mating. Several structurally related peptides stimulated CA activity to a similar extent as Manse-AT. Corpora allata activity was stimulated by a Ca2+ ionophore, A23187. A membrane-permeable Ca2+ chelator, BAPTA/AM, antagonized the stimulatory effects of Manse-AT, suggesting that Manse-AT may enhance CA activity by increasing intracellular Ca2+ concentration. Arch. Insect Biochem. Physiol. 54:121–133, 2003. © 2003 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text