Application of polymerase chain reaction to fingerprinting Aspergillus fumigatus by random amplification of polymorphic DNA
| Autor: | A P Coke, K W Loudon, J P Burnie, R C Matthews |
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| Rok vydání: | 1993 |
| Předmět: |
Microbiology (medical)
Molecular Sequence Data Mycology Polymerase Chain Reaction Aspergillus fumigatus law.invention chemistry.chemical_compound law Aspergillosis Humans Typing skin and connective tissue diseases DNA Fungal Polymerase chain reaction Genetics Polymorphism Genetic Base Sequence biology Fungi imperfecti bacterial infections and mycoses biology.organism_classification DNA Fingerprinting Molecular biology RAPD chemistry DNA profiling Evaluation Studies as Topic Restriction fragment length polymorphism DNA Research Article |
| Zdroj: | Journal of Clinical Microbiology. 31:1117-1121 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | A new method for fingerprinting Aspergillus fumigatus by random amplification of polymorphic DNA (RAPD) by using single primers with arbitrary sequences is described. Five primers were examined with 19 isolates from six patients with aspergilloma as well as with A. fumigatus NCPF 2109. Two of the primers (GCT GGT GG and GCG CAC GG, 5' to 3') gave adequate discrimination between isolates, generating five and six types, respectively. Combination of the results obtained with each of these two primers generated 12 types. This compares very favorably with immunoblot fingerprinting and XbaI-generated restriction fragment length polymorphisms on the same isolates. Typeability and reproducibility were good with RAPD, and RAPD was less labor-intensive than immunoblot fingerprinting. RAPD typing results suggested that aspergillomas sometimes contain isolates of more than one type. |
| Databáze: | OpenAIRE |
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