Multifocus structured illumination microscopy for fast volumetric super-resolution imaging
| Autor: | Ana Agostinho, Aurélie Jost, Hans Blom, Hjalmar Brismar, Marcel Müller, Kristoffer Bernhem, Linnéa Nilsson, Sara Abrahamsson, Rainer Heintzmann, Daniel C. Jans, Talley J. Lambert |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Diffraction Materials science business.industry Structured illumination microscopy Image processing 01 natural sciences Superresolution Article Atomic and Molecular Physics and Optics 010309 optics 03 medical and health sciences Biological specimen 030104 developmental biology Optics Three dimensional imaging 0103 physical sciences Microscopy Computer vision Artificial intelligence business Biological imaging Biotechnology |
| Zdroj: | Biomedical Optics Express. 8:4135 |
| ISSN: | 2156-7085 |
| DOI: | 10.1364/boe.8.004135 |
| Popis: | We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in superresolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diffraction limit. Employing multifocus diffractive optics we obtain simultaneous wide-field 3D imaging capability in the SIM acquisition sequence, improving volumetric acquisition speed by an order of magnitude. Imaging performance is demonstrated on biological specimens. (C) 2017 Optical Society of America |
| Databáze: | OpenAIRE |
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