Physiological and immune response of juvenile rainbow trout to dietary bovine lactoferrin

Autor: Peter Bossier, Syaghalirwa N.M. Mandiki, Stéphane Betoulle, Jessica Douxfils, Patrick Kestemont, Lluis Tort, Felipe E. Reyes-López, Valérie Cornet, Trinh Dinh Khuyen
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Oncorhynchus mykiss/genetics
Rainbow trout (Oncorhynchus mykiss)
Time Factors
Lymphocyte
Administration
Oral
Gene Expression
Aeromonas salmonicida
Bovine lactoferrin
Fish Diseases
Anti-Infective Agents
Immunologic
Fish Diseases/immunology
Leukocytes
Leukocytes/immunology
Gene Expression Profiling/veterinary
Disease resistance
Lactoferrin/administration & dosage
Lactoferrin
Animal Feed/analysis
Humoral
General Medicine
Gene Expression/immunology
Respiratory burst
medicine.anatomical_structure
Oncorhynchus mykiss
Administration
Gene expressions
Oral
medicine.medical_specialty
Dose-Response Relationship
Immunologic
Spleen
Aquatic Science
Biology
Anti-Infective Agents/administration & dosage
Dose-Response Relationship
03 medical and health sciences
Immune system
Internal medicine
medicine
Environmental Chemistry
Animals
Diet/veterinary
Gram-Negative Bacterial Infections/immunology
Innate immune system
Gene Expression Profiling
Monocyte
Immunity
Animal Feed
Diet
Immunity
Humoral
030104 developmental biology
Endocrinology
Humoral immunity
biology.protein
Gram-Negative Bacterial Infections
Disease Resistance/immunology
Aeromonas salmonicida/physiology
Zdroj: Fish & Shellfish Immunology. 71:359-371
ISSN: 1095-9947
1050-4648
DOI: 10.1016/j.fsi.2017.10.027
Popis: Lactoferrin, a large multifunctional glycoprotein, is involved in many physiological functions but its immunomodulatory pathways are not well characterized in fish. The objective of the present study was to investigate the temporal effect of dietary bovine lactoferrin (BLf) at low (0.1%) and high (1%) on immunological organs of rainbow trout juveniles. BLf diets did not affect specific growth rate, haematocrit, splenic index, spleen respiratory burst activity as well as humoral (mIgM) and neutrophils (MPO) gene expressions after short term - 35 days (D35) and long term nutrient test - 51 days (D51) of feeding. Both low and high BLf doses induced enhanced level of plasma alternative complement activity, plasma total immunoglobulin on D35 and D51, lymphocyte plus thrombocyte cell proportion on D35 and monocyte cell proportion in total blood leukocyte cells on D51. On D51 but not on D35, BLf diets upregulated the expression of inflammatory genes in kidney for il-1 at the low BLf dose, il-8 at both BLf doses and il-6 at the high BLf dose in spleen, and il-10 at both BLf doses in kidney. Moreover, the expression of T helper (cd4-2α; cd4-2β) genes was significantly upregulated only on D51 by both BLf doses in both spleen and kidney tissues. On D51, controls and BLf treated fish were intraperitoneally injected with A. salmonicida achromogenes. The expression of 13 immune genes was evaluated at 44 h post-injection (D54). The expression of lysozyme gene was upregulated by both BLf doses after bacterial infection both in spleen and kidney. The expression of mcsfrα (spleen) and tgf-β1 (kidney) was also modulated by both BLf doses. Low and high BLf doses enhanced disease resistance of rainbow trout juveniles with the cumulative survival rate of 36% and 38% respectively while those of the control was 19% after 14 days challenged with bacteria. The results indicate that BLf diets activated the humoral immunity, associated to blood leukocyte cells of rainbow trout after short term BLf administration, and the long term BLf administration was necessary for sensitizing other lymphoid organs such as in spleen and kidney. Only after long term test, BLf diets induced significantly higher levels of innate and adaptive immune gene expressions than those of the control. Dietary BLf activated more markedly the expression of innate immune genes than the adaptive ones; this upregulation of some immune genes could explain the high disease resistance observed in rainbow trout juveniles fed BLf.
Databáze: OpenAIRE
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