Protein aggregates as depots for the release of biologically active compounds
| Autor: | Elena M. Lyutova, Alexei S. Kasakov, Bella Gurvits, Zoya M. Bumagina, Natalya V. Artemova |
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| Rok vydání: | 2008 |
| Předmět: |
Protein Folding
Hot Temperature Light Chemistry Alcohol Dehydrogenase Biophysics Biological activity Cell Biology Protein aggregation Biochemistry In vitro Fungal Proteins Kinetics Models Chemical Dynamic light scattering Amphiphile Scattering Radiation Protein folding Amino Acid Sequence Turbidimetry Peptides Molecular Biology Peptide sequence |
| Zdroj: | Biochemical and Biophysical Research Communications. 377:595-599 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2008.10.015 |
| Popis: | Protein misfolding and aggregation is one of the most serious problems in cell biology, molecular medicine, and biotechnology. Misfolded proteins interact with each other or with other proteins in non-productive or damaging ways. However, a new paradigm arises that protein aggregation may be exploited by nature to perform specific functions in different biological contexts. From this consideration, acceleration of stress-induced protein aggregation triggered by any factor resulting in the formation of soluble aggregates may have paradoxical positive consequences. Here, we suggest that amorphous aggregates can act as a source for the release of biologically active proteins after removal of stress conditions. To address this concept, we investigated the kinetics of thermal aggregation in vitro of yeast alcohol dehydrogenase (ADH) as a model substrate in the presence of two amphiphilic peptides: Arg-Phe or Ala-Phe-Lys. Using dynamic light scattering (DLS) and turbidimetry, we have demonstrated that under mild stress conditions the concentration-dependent acceleration of ADH aggregation by these peptides results in formation of large but soluble complexes of proteins prone to refolding. |
| Databáze: | OpenAIRE |
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