Comparative evaluation of the isolation and quantification of stem cells derived from dental pulp and periodontal ligament of a permanent tooth and to assess their viability and proliferation on a platelet-rich fibrin scaffold
| Autor: | Praveen Kudva, Syed Yawer Husain, Rohit Khurana |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
CD34
Dentistry platelet-rich fibrin Andrology 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine stomatognathic system Dental pulp stem cells Medicine Periodontal fiber Viability assay Confluency mesenchymal stem cells business.industry periodontal ligament Mesenchymal stem cell 030206 dentistry Platelet-rich fibrin lcsh:RK1-715 Dental pulp stomatognathic diseases lcsh:Dentistry Periodontics Original Article Stem cell business |
| Zdroj: | Journal of Indian Society of Periodontology Journal of Indian Society of Periodontology, Vol 21, Iss 1, Pp 16-20 (2017) |
| ISSN: | 0972-124X |
| Popis: | Background: The present study aims to comparatively evaluate the isolation and quantification of stem cells derived from dental pulp and periodontal ligament of a permanent tooth and to assess their viability and proliferation on a platelet-rich fibrin (PRF) scaffold. Materials and Methods: A total of 15 systemically healthy individuals between the age group of 15–25 years requiring third molar or orthodontic premolar extractions. Teeth were extracted atraumatically and transported to the laboratory. Stem cells were isolated from dental pulp and periodontal ligament. After attaining more than 90% confluency by the 7th day, these cells were tested for their viability and characterization. Stem cells were also incubated with PRF and viability was assessed on the 7th day. Results: The mean number of cell for dental pulp stem cells (DPSCs) and periodontal ligament stem cell (PDLSC) was statistically insignificant (P > 0.05). The mean live cell viability was compared between DPSC (98.07%) and PDLSC (98%). Both DPSC and PDLSC showed a high percentage of expression of CD73 markers, 30.40% and 29.80%, respectively. However, DPSCs and PDLSCs lacked expression of CD34 expressing only 3.47% and 3.53%, respectively. PRF membrane as a scaffold exhibited no cytotoxic effects on DPCS's or PDLSC's. The cell viability of cells cultured with PRF was statistically insignificant (P > 0.05) when compared to the cells cultured with culture media. Conclusion: The study thus indicates that dental pulp and periodontal ligament are both rich sources of mesenchymal stem cells and can be successfully used for obtaining stem cells. PRF exhibits no cytotoxic effects on the cells and can be used in conjunction with dental stem cells. |
| Databáze: | OpenAIRE |
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