Changes in flavonoid content and tyrosinase inhibitory activity in kenaf leaf extract after far-infrared treatment
| Autor: | Ho Sik Rho, Dong Ha Cho, Cheng Wu Jin, Amal Kumar Ghimeray, Bum Chun Lee, Myung Kyoo Kim, Soo Mi Ahn |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Infrared Rays
Tyrosinase Clinical Biochemistry Flavonoid Pharmaceutical Science Biochemistry chemistry.chemical_compound Drug Discovery Kaempferitrin Proanthocyanidins Afzelin Food science Kaempferols Catechol oxidase Molecular Biology chemistry.chemical_classification Flavonoids biology Monophenol Monooxygenase Plant Extracts Organic Chemistry biology.organism_classification Kenaf Plant Leaves Aglycone chemistry Hibiscus Mannosides biology.protein Molecular Medicine Kaempferol |
| Zdroj: | Bioorganicmedicinal chemistry letters. 20(24) |
| ISSN: | 1464-3405 |
| Popis: | The tyrosinase inhibitory activity of ethanolic extract of kenaf (Hibiscus cannabinus L.) leaf was evaluated before and after subjecting it to far-infrared (FIR) irradiation. The main component of the extract was analyzed as kaempferitrin (kaempferol-3,7-O-α-dirhamnoside). Prior to FIR irradiation, no inhibitory activity of the extract was detected in a tyrosinase assay. However, after FIR irradiation for 1h at 60°C, significant tyrosinase inhibitory activity (IC(50)=3500 ppm) was observed in it. In HPLC analysis, derhamnosylation products (kaempferol, afzelin, and α-rhamnoisorobin) were detected. The inhibitory activity may be due to the existence of derhamnosylation products. This study demonstrated that FIR irradiation can be used as a convenient tool for deglycosylation of flavonoid glycoside. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect