Production of Cryoprecipitate of Intermediate Purity in a Closed System Thaw-Siphon Process

Autor:	Ernest C Mason, Duncan S. Pepper, Brenda Doreen Griffin
Rok vydání:	1981
Předmět:	Chromatography Chemistry Yield (chemistry) Cryoprecipitate Mean value Blood plasma fractionation Hematology Blood bank Total protein
Zdroj:	Thrombosis and Haemostasis. 46:543-546
ISSN:	2567-689X 0340-6245
DOI:	10.1055/s-0038-1653406
Popis:	SummaryThe thaw-siphon procedure for routine production of cryoprecipitate is described briefly, and also its extension to a reproducible procedure for production of cryoprecipitates of intermediate purity. For the latter, the average yield of factor VIII is 60% of that in the prefrozon plasma and approximately 30% is present in the supernatant plasma. The product contains 0.53 units of VIII:C per mg of total protein (of which about 70% is clottable). The mean value of the ratio VIII:C/VIII:RAG is about 0.7. The yield and purity of the product compare favourably with those reported for intermediate purity factor VIII concentrates produced by large-scale plasma fractionation laboratories. The procedure offers an alternative pathway to the production of factor VIII concentrate of intermediate purity and can be operated in any blood bank with a minimum of equipment.
Databáze:	OpenAIRE
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