PCR-Genosensor Rapid Test for DetectingSalmonella

Autor:	Salvador Alegret, Jordi Barbé, M. Isabel Pividori, Arben Merkoçi
Rok vydání:	2003
Předmět:	Electrode material Salmonella Chromatography Chemistry Electrochemistry medicine Amplicon medicine.disease_cause Molecular biology Analytical Chemistry Graphite epoxy composite
Zdroj:	Electroanalysis. 15:1815-1823
ISSN:	1521-4109 1040-0397
Popis:	A PCR-genosensor based assay for detecting the IS200 DNA sequence specific to Salmonella spp is reported. This rapid test is based on PCR, where amplicon detection is achieved electrochemically using GEC (graphite-epoxy composite) genosensor. The amplicon is immobilized onto GEC electrodes by simple dry-adsorption and the detection is carried out using an enzymatic labeling system. Results demonstrate that this new electrochemical genosensor fulfils the requirements desired for these devices: easy preparation – as dry-adsorption of DNA is very simple –, robustness, sensitivity, low cost, simple use and fast response. Additionally, the assay can be produced as a kit format, increasing its commercial potentiality. Also, the electrode material can be implemented for screen-printing procedures for the mass production of genosensors.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ff1c6e94c27a51a58d63c2bcc4b5c131 https://doi.org/10.1002/elan.200302764 Zobrazit plný text záznamu Plný text