Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet
| Autor: | Jun-Ming Liu, Xiaoli Ni, Yan-Qing Guan, Xin Wang, Zhibin Li, Ami Yang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Materials science
Polymers and Plastics Mechanical Engineering Metals and Alloys High-performance liquid chromatography Lower critical solution temperature Contact angle chemistry.chemical_compound chemistry Chemical engineering Mechanics of Materials Self-healing hydrogels Polymer chemistry Materials Chemistry Ceramics and Composites Copolymer Polystyrene Fourier transform infrared spectroscopy Acrylic acid |
| Zdroj: | Journal of Materials Science & Technology. 26:1119-1126 |
| ISSN: | 1005-0302 |
| DOI: | 10.1016/s1005-0302(11)60011-x |
| Popis: | In this study, acrylic acid (AA) and 4-azidoaniline were used to modify poly (N-isopropylacrylamide) (NI-PAAm) in order to fabricate temperature-responsive surface for corneal epithelia cell adhesion and detachment. First, NIPAAm was copolymerized with acrylic acid. Then, the copolymer was coupled with azidoaniline to synthesize AzPhPIA, derivative of p(NIPAAm-co-AA), which possesses both thermo- and photo-sensitivities. Second, the synthesized copolymer was characterized by high performance liquid chromatography (HPLC), Fourier transform infrared (FTIR) and a CHN analyzer. The thermo-sensitivity was characterized by temperature reducing experiment, contact angle measurement and low critical solution temperature (LCST) testing. Third, the derivatized copolymer was immobilized by photolithography on a polystyrene plate, and then the surface characterization of AzPhPIA-coated polystyrene plate (PSt) was measured by electron spectroscopy for chemical analysis (ESCA). The thermo-sensitivity and cytocompatibility of the AzPhPIA-coated PSt were investigated by corneal epithelial cells culture. The results revealed that the AzPhPIA-coated PSt exhibited good cytocompatibility and cell detachability when temperature decreased. |
| Databáze: | OpenAIRE |
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