Oxidative Degradation of Diazinon byDrosophila:Metabolic Changes Associated with Insecticide Resistance and Induction
| Autor: | Jean-Baptiste Bergé, A. Brun, E. Pisani-Borg, Marcel Amichot, André Cuany, Didier Fournier |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Diazinon Clofibrate biology Health Toxicology and Mutagenesis Metabolite General Medicine Metabolism Hydroxylation chemistry.chemical_compound Metabolic pathway Enzyme chemistry Biochemistry Enzyme inhibitor medicine biology.protein Agronomy and Crop Science medicine.drug |
| Zdroj: | Pesticide Biochemistry and Physiology. 54:56-64 |
| ISSN: | 0048-3575 |
| Popis: | Using [ 14 C]diazinon, it was found that four molecules accounted for most of the metabolites after in vitro or in vivo incubation with Drosophila melanogaster. RalDDT R , an insecticide-resistant strain of Drosophila, produced higher amounts of each metabolite than Canton S , a susceptible strain. However, the degradative metabolic pathway giving hydroxydiazinon and pyrimidine was twofold faster than the activating metabolic pathway giving hydroxydiazoxon and diazoxon. Clofibrate and phenobarbital increased the metabolism of diazinon in induced, susceptible flies. Phenobarbital was more potent than clofibrate in stimulating the hydroxylation of diazinon. Kinetic analysis of in vivo inhibition of brain acetylcholinesterase by diazinon or hydroxydiazinon demonstrated that degradation of diazinon, especially in RalDDT R , was delayed compared to the degradation of hydroxydiazinon. Varying levels of diazinon tolerance among Drosophila strains may be explained by differential metabolic pathways. The most conclusive result was a strong interaction between tolerance and the rate of formation of hydroxylated derivatives. |
| Databáze: | OpenAIRE |
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