Induction of Oral Tolerance in TGF-β1 Null Mice
| Autor: | K. Siobhan Barone, Dana D. Tolarova, Ilona Ormsby, Thomas Doetschman, J. Gabriel Michael |
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| Rok vydání: | 1998 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 161:154-160 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Previous studies have suggested that oral tolerance induction by low doses of Ag is mediated by inhibitory cytokines, particularly TGF-β1. To examine the roles of TGF-β1 and other inhibitory cytokines in the induction of oral tolerance, TGF-β1 null mice and controls were gavaged with 10 to 20 mg (high dose) or 1 mg (low dose) of OVA for 3 days. After immunization with OVA, the in vitro proliferative response of OVA-specific popliteal lymph node cells was assessed. Lymphocytes from all TGF-β1 null mice fed high doses of OVA exhibited highly significant suppression compared with controls. A weaker, but still significant, suppression was observed in lymphocytes from the majority of TGF-β1 null mice fed low doses of OVA. In addition, supernatants from these lymphocytes exhibited lower levels of IL-4, IL-10, and IFN-γ than those from water-fed control animals. These results indicate that while TGF-β1 may play a role in suppression, inhibitory cytokines are not the exclusive mechanism by which low dose oral tolerance is induced. |
| Databáze: | OpenAIRE |
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