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Background: Acinetobacter species are very common in hospital acquired infections; Carbapenems resistant species become common in hospital and represent a major health problem. Methodology: Our study was carried on 150 patients from ICU; we compared different phenotypic methods and PCR for detection of carbapenems resistance in Acinetobacters. Results: 88.9% of resistant Acinetobacters were detected by Kirby Bauer Disk diffusion method, 54.2% by Modified Hodge test, 70.8 % by EDTA disc synergy, 85.4% by Amp C disk test, 33.3% by PCR bla-IMP and 12.5% by PCR blaVIM. Conclusion: We recommended for detection of carbapenems resistance in Acinetobacters the phenotypic methods as they are rapid, accurate methods and less costly than PCR. Note: bla-IMP gene (NCBI GenBank Nucleotide accession # LC103138.1); bla-VIM gene (NCBI GenBank Nucleotide accession # MT185944.1 GenBank accession number S71932 |
