Abstract P128: Sexual Dimorphisms In Perivascular Adipose Tissue In A Novel Mouse Model Of Diet-Induced Metabolic Syndrome
| Autor: | Vivian Tran, Holly Brettle, Henry Diep, Quynh Nhu Dinh, Christopher G Sobey, Grant Drummond, Antony Vinh, Maria Jelinic |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Hypertension. 79 |
| ISSN: | 1524-4563 0194-911X |
| DOI: | 10.1161/hyp.79.suppl_1.p128 |
| Popis: | Metabolic syndrome (MetS) is a complex multifactorial disease that increases cardiovascular risk. Vascular dysfunction associated with MetS is strongly influenced by perivascular adipose tissue (PVAT) which acts as a reservoir for immune cells. Sexual dimorphisms of PVAT pathophysiology are poorly understood due to a lack of clinically relevant animal models of MetS that show metabolic disturbances in males and females. Using a new diet-induced model of MetS pathophysiology in both sexes, we characterised the aortic leukocyte profile and determined PVAT influence on vascular function. Six-week-old male and female C57BL/6 mice were fed either a high-fat diet (43% kcal) with high sugar and salt (10% high fructose and 0.9% NaCl; HFSS) in drinking water, or normal control diet (NCD) for 10 weeks. Physiological parameters were measured fortnightly. At endpoint, aorta with PVAT intact was isolated for leukocyte enumeration (flow cytometry) or for in vitro vascular reactivity (± PVAT; wire myography). Weight gain (NCD M: 29.3±0.5 g vs. MetS M: 37.5±1.0 g; NCD F: 23.6±0.7 g vs. MetS F: 28.9±1.2 g), fasting blood glucose (NCD M: 9.5±0.4 mmol/L vs. MetS M: 11.3 mmol/L; NCD F: 8.9±0.3 mmol/L vs. 10.4±0.5 mmol/L), blood cholesterol (NCD M: 125.0±3.0 mg/dL vs. MetS M: 177.5±6.5 mg/dL; NCD F: 96.7±2.9 mg/dL vs. MetS F: 130.7±3.4 mg/dL) and systolic blood pressure (NCD M: 121.0±1.8 mmHg vs. MetS M: 132.0±2.1 mmHg; NCD F: 122.3±2.2 mmHg vs. MetS F: 133.6±2.7 mmHg) were increased in both sexes of HFSS-fed mice (Phi+ ; M: 3.3±0.5 x 10 3 cells/aorta vs F: 1.6±0.4 x 10 3 cells/aorta) and neutrophils (Ly6G + ; M: 0.9±-0.2 x 10 3 cells/aorta vs F: 0.3+/0.1 x 10 3 cells/aorta); P |
| Databáze: | OpenAIRE |
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