An exoantigen test for the rapid identification of medically significantFusariumspecies

Autor: Leo Kaufman, E.A. Ambrosie, N. Moledina, Tony Panter, Arvind A. Padhye, A.S. Sekhon, Richard C. Summerbell
Rok vydání: 1995
Předmět:
Zdroj: Medical Mycology. 33:287-289
ISSN: 1460-2709
1369-3786
DOI: 10.1080/02681219580000591
Popis: The accurate identification of Fusarium species can take 2–3 weeks. Preliminary exoantigen studies indicate that a mature culture suspected of being a Fusarium species may be immunologically identified 48 h after receipt. Exoantigen extracts of 10-day-old slant cultures of Fusarium chlamydosporum, Fusarium moniliforme ( = Fusarium verticilloides), Fusarium oxysporum, Fusarium proliferatum and Fusarium solani and partially purified reference homologous and heterologous shake culture extracts (6-week-old) were reacted against rabbit anti-F. chlamydosporum, F. moniliforme, F. oxysporum, F. proliferatum and F. solani sera, in a micro-immunodiffusion procedure. The results indicated that all the strains belonging to a given species produced 1–3 bands of identity only when tested against its homologous antiserum and reference antigen. No cross-reactions were observed with the heterologous antisera. Furthermore, extracts from isolates of Fusarium dimerum, Fusarium equiseti, Fusarium roseum complex, Acremonium sp...
Databáze: OpenAIRE