Unusual localization of nod and nif genes in Rhizobium leguminosarum bv. viciae
| Autor: | Sylvie-Isabelle Mazurier, Gisèle Laguerre |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Genetics
Immunology food and beverages Nif gene General Medicine biochemical phenomena metabolism and nutrition Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Rhizobium leguminosarum Homology (biology) genomic DNA Plasmid Extrachromosomal DNA Botany medicine bacteria Replicon Molecular Biology Gene |
| Zdroj: | Canadian Journal of Microbiology. 43:399-402 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m97-056 |
| Popis: | Genomic DNA from seven strains of Rhizobium leguminosarum bv. viciae isolated from nodules of field-grown lentils showed homology to nod and nif gene probes, whereas plasmid DNA did not hybridize with these probes. The results suggest that symbiotic genes could be located on the chromosome or perhaps on a very large plasmid that could not be resolved in Eckhardt gels. Each strain contained one plasmid that hybridized with a pSym isolated from a R. leguminosarum strain of the same field population. This finding led us to hypothesize that the nod and nif genes of the seven strains might have originated from a Sym plasmid and have been integrated into another replicon. The ability to nodulate vetch was confirmed for all of the seven strains. Thus, wild strains of R. leguminosarum bv. viciae that nodulate vetch carry nod and nif genes either on the chromosome or on an extrachromosomal replicon of size much larger than the pSyms hitherto described.Key words: Rhizobium leguminosarum, nod genes, nif genes, chromosome, symbiotic plasmid, megaplasmid. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|