New morphological criteria and molecular characterization of black aspergilli aggregate from corn, sorghum and wheat grains
| Autor: | Thanaa A. Maghraby, Eman G A M El-Dawy, Lamiaa H A Hamza, Youssuf A. Gherbawy |
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| Rok vydání: | 2020 |
| Předmět: |
Ochratoxin A
0303 health sciences biology 030306 microbiology food and beverages General Medicine Sorghum biology.organism_classification Biochemistry Microbiology Conidium 03 medical and health sciences chemistry.chemical_compound Horticulture chemistry Genetics Malt extract agar Mycotoxin Molecular Biology Ochratoxin 030304 developmental biology |
| Zdroj: | Archives of Microbiology. 203:355-366 |
| ISSN: | 1432-072X 0302-8933 |
| DOI: | 10.1007/s00203-020-02024-5 |
| Popis: | Corn, sorghum and wheat grains are used as livestock feed in the world. Identification of black aspergilli associated with these grains is necessary to make sure of the safety of the grains because its occurrence is an indicator of mycotoxin production. Forty-five isolates were isolated from the samples collected from Upper Egypt’s markets and identified morphologically based on colony color, conidia, stipe and vesicle size and molecularly by using β-tubulin and calmodulin genes. Isolates were divided into 30 strains of Aspergillus welwitschiae and 15 strains of A. niger. We have found new criteria in the morphological identification of A. welwitschiae as its colony color was black to brown with yellow edge, but in A. niger was black with white edge, also A. welwitschiae sometimes produced finely-to-distinctly roughened brownish conidia on malt extract agar (MEA) media. Thirteen isolates of A. welwitschiae and six of A. niger were recognized as potential producers for ochratoxin A. |
| Databáze: | OpenAIRE |
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