Effects of electroacupuncture of different frequencies on electromyography, NOS and ICC of colon in rats with slow transit constipation

Autor: Yu-lei Liang, Yan-Hui Sun, B. T. Liu, Hui-zhen Zhang, Xiao-Feng Li, Xuan-Ping Zhang, Bo Zhao, Yan-li Liu, Fei Gao, Shen Zhang, Chun-Sheng Jia
Rok vydání: 2020
Předmět:
Zdroj: Journal of Acupuncture and Tuina Science. 18:10-15
ISSN: 1993-0399
1672-3597
Popis: To observe the effects of electroacupuncture (EA) of different frequencies on transmission function, electromyography, nitric oxide synthase (NOS) content and interstitial cells of Cajal (ICC) expression of colon in rat models with slow transit constipation (STC). Of the 50 healthy male Wistar rats, 10 were randomly selected as a normal group and fed with ordinary diet, and the remaining 40 rats were fed with the diet added with the compound diphenoxylate at a dose of 8 mg/(kg·bw) each day for continuous 120 d. The 40 successfully established STC rat models were randomly divided into a model group, a low-frequency EA group (2 Hz), a high-frequency EA group (100 Hz), and a variable-frequency EA group (2 Hz/100 Hz), with 10 rats in each group. Rats in the normal and the model groups were not given any treatment; the low-frequency EA and the high-frequency EA groups were given EA at Tianshu (ST 25), Zusanli (ST 36) and Zhigou (TE 6) with continuous wave at the designated frequency, and the variable-frequency EA group received sparse-dense wave (2 Hz/100 Hz) EA at the same acupoints, once a day for a total of 15 d. After treatment, the colonic transmission function, electromyography, NOS content and ICC expression (calculated by the difference in the area of the C-kit positive cells) of the rats in each group were measured. For the colonic transmission function, compared with the normal group, the first black stool excretion durations of rats in the other groups were significantly prolonged (all P
Databáze: OpenAIRE
Externí odkaz: