Structural and biochemical characterization of native and recombinant single insulin-like growth factor-binding domain protein (SIBD-1) from the Central American Hunting Spider Cupiennius salei (Ctenidae)

Autor:	Christine Widmer, Ulrich Baumann, Lucia Kuhn-Nentwig, Tommy Baumann, Claudia Bühr, Urs Kämpfer, Johann Schaller, Stefan Schürch, Christian Trachsel
Rok vydání:	2012
Předmět:	biology Protein domain Anatomy Protein superfamily biology.organism_classification Biochemistry Cupiennius salei Insulin-like growth factor-binding protein Protein structure Structural Biology biology.protein Insulin-like growth factor binding Molecular Biology Peptide sequence Binding domain
Zdroj:	Proteins: Structure, Function, and Bioinformatics. 80:2323-2329
ISSN:	0887-3585
DOI:	10.1002/prot.24119
Popis:	Cupiennius salei single insulin-like growth factor binding domain protein (SIBD-1) is an 8.6 kDa Cys-, Pro-, and Gly-rich protein, discovered in the hemocytes of the Central American hunting spider Cupiennius salei. SIBD-1 exhibits high sequence similarity to the N-terminal domain of the insulin-like growth factor-binding protein superfamily and has been reported to play an important role in the spider's immune system. Here, the recombinant expression and the elucidation of the three-dimensional structure of recombinant SIBD-1 and the characterization of the sugar moiety at Thr2 of native SIBD-1 is described in detail.
Databáze:	OpenAIRE
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