Absorption of Thyrotropin-Releasing Hormone in Rats Using a Mucoadhesive Buccal Patch
| Autor: | Padmanabh Pravinchandra Bhatt, Cheng Li, V. A. Raul, R. L. Koch, Thomas P. Johnston |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Pharmacology
endocrine system endocrine system diseases Organic Chemistry Pharmaceutical Science Thyrotropin-releasing hormone Buccal administration Biology Peptide hormone High-performance liquid chromatography Dosage form In vivo Drug Discovery Liberation Incubation hormones hormone substitutes and hormone antagonists |
| Zdroj: | Drug Development and Industrial Pharmacy. 23:239-246 |
| ISSN: | 1520-5762 0363-9045 |
| Popis: | Mucoadhesive buccal patches were evaluated in vitro and in vivo using rats for release of thyrotropin-releasing homne (TRH). TRH (10% w/w) was incorporated into mucoadhesive buccal patches that were custom coformulated with silicone and organic polymers (Dow Coming, Midland, MI) and its release profile was characterized in vitro using a modified Franz diffusion cell. TRH released into pH = 7.0 phosphate buffered saline at 37°C under sink conditions was detected using high-performance liquid chromatography (HPLC). Release of TRH in vitro from the buccal patches was rapid during the first 2 hr, with 51% of the total amount of TRH incolporated into the patches released after 24 hr. HPLC analysis indicated that TRH extracted from buccal patches thermally stressed at 40°, 55°, and 70°C showed negligible degradation after 6 months. In contrast, an aqueous TRH solution stored at 70°C showed degradation of TRH as soon as 10 days following incubation at this temperature. TRH patches placed on the buccal mu... |
| Databáze: | OpenAIRE |
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