Optimization of nutritional conditions for nattokinase production by Bacillus natto NLSSE using statistical experimental methods

Autor:	Zhiya Ma, Huizhou Liu, Tian-Shi Chang, Jianmin Xing, Junguo Liu
Rok vydání:	2005
Předmět:	Central composite design Chemistry Bacillus natto Fractional factorial design Bioengineering Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound Fibrinolytic enzyme Yeast extract Food science Experimental methods Nattokinase
Zdroj:	Process Biochemistry. 40:2757-2762
ISSN:	1359-5113
DOI:	10.1016/j.procbio.2004.12.025
Popis:	Nattokinase is a potent fibrinolytic enzyme. The composition of the medium for the production of nattokinase was optimized with a stepwise strategy. Effects of various nitrogen and carbon sources were investigated. Fractional factorial design (FFD) was applied to elucidate the key ingredients in the media and the results indicated that the soy peptone, yeast extract and calcium chloride have a significant effect on nattokinase production. Central composite design (CCD) was employed to search for the optimal concentration of the three key components and the experimental results were fitted with a second-order polynomial model at 98% level ( P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f4fb648a37834dc664ce28bf92490d22 https://doi.org/10.1016/j.procbio.2004.12.025 Zobrazit plný text záznamu Full Text from ScienceDirect