140 A comparison of in vitro embryo production between heifers and lactating Holstein donors without superstimulation
| Autor: | I. Rivelli, Elena Silva, L. F. Nasser, F. Rochelle, P. V. Marchioretto, Marcello Rubessa, L. M. Naves, M. B. Wheeler, R. Della Méa, M. K. Sermersheim |
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| Rok vydání: | 2019 |
| Předmět: |
Estrous cycle
animal structures animal diseases Semen Embryo culture Reproductive technology Biology Endocrinology Animal science medicine.anatomical_structure Human fertilization Reproductive Medicine Lactation Reproductive biology Genetics medicine Animal Science and Zoology Blastocyst Molecular Biology Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 31:195 |
| ISSN: | 1031-3613 |
| DOI: | 10.1071/rdv31n1ab140 |
| Popis: | Bovine oocyte donor age seems to have an important role on in vitro embryo production. Therefore, the aim of this study was to compare cumulus-oocyte complex (COC) recovery, cleavage, and blastocyst rates between heifers and lactating Holstein donors. A total of 89 animals (heifers: n=60, 11 to 17 months of age; lactating cows: n=29, 60 to 180 days in milk) were used in this experiment. Ovum pickup (Day −1) was performed on a random day of the oestrous cycle without superstimulation by a single technician. Conventional or sexed semen were used for IVF (Day 0). Cleavage and blastocyst rates were evaluated on Days 3 and 7, respectively. Cleavage was determined when structures had 4 or more cells and blastocyst rate included grades I and II blastocysts. Continuous data were analysed by t-test, and binomial data were analysed by Fischer’s exact test. A total of 1,289 COC were recovered from lactating cows (n=509) and heifers (n=777). Total and viable COC per donor were higher (P0.05) in lactating cows (64.04%; 326/509) and heifers (60.87%; 473/777). Cleavage rate was higher (P0.05) with conventional and sexed semen in heifers (conventional=37.68%; 52/138; sexed=46.63%; 298/639) and cows (conventional=55.05%; 49/89; sexed=59.52%; 250/470). Blastocyst rate was higher (P0.05) in blastocyst rate between conventional (24.71%; 22/89) and sexed semen (18.8%; 79/420) in lactating cows. Blastocyst/cleaved structures ratio was higher (P |
| Databáze: | OpenAIRE |
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