Effect of chemical modification strategy on the characteristics of copper-67-Labeled immunoconjugates, Part I: Immunoreactivity
| Autor: | Robert L. Vessella, Janet A. Mercer-Smith, Jeanette C. Roberts, David K. Lavallee, Mahesh K. Bhalgat |
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| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Drug Delivery. 4:1-12 |
| ISSN: | 1521-0464 1071-7544 |
| DOI: | 10.3109/10717549709033181 |
| Popis: | The synthetic porphyrins N-benzyl-5,10,15,20-tetrakis(4-carboxyphenyl)porphine (N-bzHTCPP) and N-4-nitrobenzyl-5-(4-carboxyphenyl)-10,15,20-tris(4-sulfophenyl)porphine (NbzHCS3P) represent excellent radiocopper chelating agents that may find utility in antibody-mediated diagnosis and/or therapy. Detailed analyses were performed to explore the effect of the chemical modification strategy on the characteristics of immunoconjugates prepared from the anti—renal cell carcinoma monoclonal antibody A6H and N-bzHCS3P. The parameters included in the study were antibody type [intact A6H and two of its fragments, half A6H and A6H-F(ab’)2], chemical linkage type and site, the presence or absence of intermediate linker molecules, and the nature of the chemical modification steps employed. The immunoconjugate synthesized by directly coupling N-bzHCS3P to whole antibody retained 75–85% of the immunoreactivity of the unmodified antibody. In general, however, immunoconjugates prepared using the fragments or the in... |
| Databáze: | OpenAIRE |
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