| Popis: |
A validated reversed-phase high-performance liquid chromatographic (HPLC) procedure for the analysis of 17β-estradiol-3-phosphate is reported. In the development of this assay, several factors were evaluated including buffer ionic strength, mobile phase pH, ion-pairing concentration, organic composition, and column type. The described method is rapid and coupled with standard HPLC procedures leads to a selective, accurate, and reproducible assay. The peak area versus 17β-estradiol-3-phosphate concentration is linear over the range of 0.1–100 μg/mL, with a detection limit of 0.02 μg/mL. |
